FlyBase Allele Report: Dmel\Trm7-32[K28A]

General Information

Symbol

Dmel\Trm7-32^K28A

Species

D. melanogaster

Name

FlyBase ID

FBal0359010

Feature type

allele

Associated gene

Dmel\Trm7-32

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Angelova et al., 2020)

Progenitor genotype

Cytology

Description

Amino acid replacement: K28A.

(Angelova et al., 2020)

Substitution of a conserved lysine at position 28 in the predicted catalytic domain with alanine, resulting in a catalytically dead mutant protein.

(Angelova et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3R:17,040,928..17,040,929 [+]

Nucleotide change:

AA17040928GC

Amino acid change:

K28A | Trm7-32-PA

Reported amino acid change:

K28A

(Angelova et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of non-syndromic X-linked intellectual disability 9

CEC

(Brazane et al., 2023)

CEC with Trm7-34^e02001

(Brazane et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage

(Angelova et al., 2020)

abnormal locomotor behavior | adult stage

(Angelova et al., 2020)

short lived

(Angelova et al., 2020)

viable

(Angelova et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

Trm7-32^K28A homozygotes survive until adulthood under standard conditions, without major growth defects or significant developmental delays, but adults are sluggish and short lived. However, they show increased viral load upon infection with Drosophila C Virus, as compared to controls.

(Angelova et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Trm7-32^K28A has short lived phenotype, non-enhanceable by Trm7-34^e02001/Trm7-34^e02001

(Angelova et al., 2020)

Trm7-32^K28A has abnormal locomotor behavior | adult stage | recessive phenotype, non-enhanceable by Trm7-34^e02001/Trm7-34^e02001

(Angelova et al., 2020)

NOT suppressed by

Statement

Reference

Trm7-32^K28A has short lived phenotype, non-suppressible by Trm7-34^e02001/Trm7-34^e02001

(Angelova et al., 2020)

Trm7-32^K28A has abnormal locomotor behavior | recessive | adult stage phenotype, non-suppressible by Trm7-34^e02001/Trm7-34^e02001

(Angelova et al., 2020)

NOT Enhancer of

Statement

Reference

Trm7-32^K28A/Trm7-32^K28A is a non-enhancer of abnormal locomotor behavior | adult stage | recessive phenotype of Trm7-34^e02001

(Angelova et al., 2020)

NOT Suppressor of

Statement

Reference

Trm7-32^K28A/Trm7-32^K28A is a non-suppressor of abnormal locomotor behavior | recessive | adult stage phenotype of Trm7-34^e02001

(Angelova et al., 2020)

Other

Statement

Reference

Trm7-32^K28A, Trm7-34^e02001 has viable phenotype

(Angelova et al., 2020)

Trm7-32^K28A, Trm7-34^e02001 has decreased body size phenotype

(Angelova et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Trm7-34^e02001, Trm7-32^K28A double homozygotes show a measurable reduction in size and weight when compared to controls.

(Angelova et al., 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG5220^K>A

(Angelova et al., 2020)

Trm7-32^K28A

dTrm7_32^*

(Molla-Herman et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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