FB2026_02 , released June 18, 2026
Allele: Dmel\TrpA1KO
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General Information
Symbol
Dmel\TrpA1KO
Species
D. melanogaster
Name
FlyBase ID
FBal0359455
Feature type
allele
Associated gene
Dmel\TrpA1
Associated Insertion(s)
TI{TI}TrpA1KO
Carried in Construct
Also Known As
TrpA1-KO
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Gu et al., 2019)
Mutagen
CRISPR/Cas9
(Gu et al., 2019)
gene targeting by homologous recombination
(Gu et al., 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}TrpA1KO
Cytology
Description

The entire TrpA1 gene has been deleted. A single attP site and a single loxP site have been inserted in place of the deleted sequence.

(Gu et al., 2019)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
complex_substitution
3L:8,894,338..8,905,731 [-]
Comment:

The entire TrpA1 gene has been deleted and replaced with a single attP site and a single loxP site.

(Gu and Xiang, 2020.6.27)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      TrpA1KO third instar larvae exhibit significantly reduced rolling in response to heat stimulation and significantly diminished writhing in response to exposure to UVC light or AITC (irritant chemical) compared to controls.

      Expression of TrpA1UAS.C.cGa or TrpA1UAS.D.cGa under the control of Scer\GAL4ppk.PG in the TrpA1KO background leads to decreased heat threshold of rolling behavior in third instar larvae compared to controls.

      (Gu et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TrpA1-KO
      (Gong et al., 2022, Gu et al., 2022, Gu et al., 2019)
      TrpA1KO
      (Gu and Xiang, 2020.6.27)
      trpA1-KO
      (Thakur et al., 2025)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)