FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\twyGFP
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General Information
Symbol
Dmel\twyGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0360808
Feature type
allele
Associated gene
Dmel\twy
Associated Insertion(s)
Carried in Construct
P{twy.GFP}
Also Known As
FBF1-GFP
Key Links
Transgenic product class
genomic_DNA
(Hou et al., 2020)
wild_type
(Hou et al., 2020)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Hou et al., 2020)
wild_type
(Hou et al., 2020)
Mutagen
in vitro construct
(Hou et al., 2020)
Progenitor genotype
Carried in construct
P{twy.GFP}
Cytology
Description

The coding and upstream regulatory sequences of CG5964 (amplified from genomic DNA) are cloned in frame with a downstream GFP tag.

(Hou et al., 2020)
Allele components
Component
Use(s)
Regulatory region(s)
twy
Encoded product / tool
twy
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      pupal stage
      scolopidial cilium
      (Hou et al., 2023)
      antennal segment 3 sense organ
      (Hou et al., 2023)
      Johnston organ
      (Hou et al., 2023)
      adult stage
      scolopidial cilium
      (Hou et al., 2023)
      antennal segment 3 sense organ
      (Hou et al., 2023)
      Johnston organ
      (Hou et al., 2023)
      adult stage | male
      spermatocyte | restricted
      (Hou et al., 2023)
      spermatid | restricted
      (Hou et al., 2023)
      Additional Information
      Statement
      Reference

      twy protein localizes at the ciliary base in both ciliated chordotonal organs and spermatocytes. In spermatocytes, it localizes to centrioles just below the transition zone and at the base of both spermatocyte primary-like cilia and spermatid ciliary caps, forming ringlike structures surrounding microtubule doublets.

      (Hou et al., 2023)
       
      Marker for
      Reflects expression of
      twy
      (Hou et al., 2023)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG5964GFP
      twyGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)