FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\wamKO
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General Information
Symbol
Dmel\wamKO
Species
D. melanogaster
Name
FlyBase ID
FBal0361910
Feature type
allele
Associated gene
Dmel\wam
Associated Insertion(s)
TI{TI}wamKO
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Bauerly et al., 2020)
Mutagen
cre recombinase
(Bauerly et al., 2020)
Progenitor genotype
TI{TI}wamKO-mc
(Bauerly et al., 2020)
Associated Insertion(s)
TI{TI}wamKO
Cytology
Description

The loxP cassette present in TI{TI}wamKO-mc has been excised, removing the Disc\RFPmCh.Ubi marker. This leaves a single loxP site inserted in place of the endogenous wam locus (2.1kb of genome sequence is deleted, removing the entire wam transcription unit).

(Bauerly et al., 2020)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3R:23,286,069..23,288,151 [-]
Comment:

The entire wam locus is deleted and a single loxP site is present in its place.

(Bauerly et al., 2020)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In wamKO homozygotes, mature sperm exhibit loss of flagellar motility and fail to fully individualize; seminal vesicles are devoid of sperm; sperm vesicle area progressively reduces in size; basal end of the testes exhibit an enlargement; individualization complexes appear abnormal; sperm flagella lack outer dynein arms on the microtubule doublets along the length of the axoneme; 64-cell cyst cell nuclei are reduced in size; mitochondria fail to properly align during meiosis; small clusters of unincorporated mitochondria are observed adjacent to the nebenkern; and mitochondrial derivatives are smaller when compared to control testes.

      (Bauerly et al., 2020)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      wamKO has male semi-fertile | dominant phenotype, non-enhanceable by bb8MB10362

      (Bauerly et al., 2020)
      NOT suppressed by
      Statement
      Reference

      wamKO has male semi-fertile | dominant phenotype, non-suppressible by bb8MB10362

      (Bauerly et al., 2020)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      wamKO
      (Bauerly et al., 2020)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)