FlyBase Allele Report: Dmel\bb8[MB10362]

General Information

Symbol

Dmel\bb8^MB10362

Species

D. melanogaster

Name

FlyBase ID

FBal0322783

Feature type

allele

Associated gene

Dmel\bb8

Associated Insertion(s)

Mi{ET1}bb8^MB10362

Carried in Construct

Also Known As

bb8^ms

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Vedelek et al., 2016)

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}bb8^MB10362

Cytology

Description

Mi{ET1} insertion in the first exon.

(Vedelek et al., 2016)

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:23,289,953..23,289,953 [+]

(Vedelek et al., 2016, Bellen, 2009.5.28)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male fertile

(Bauerly et al., 2020)

male sterile

(Vedelek et al., 2016)

male sterile (with Df(3R)BSC619)

(Vedelek et al., 2016)

male sterile (with Df(3R)Exel9012)

(Vedelek et al., 2016)

male sterile | recessive

(Bauerly et al., 2020)

Phenotype Manifest In

mitochondrial derivative | adult stage | male

(Vedelek et al., 2016)

mitochondrion | adult stage | male

(Vedelek et al., 2016)

spermatid

(Vedelek et al., 2016)

sperm individualization complex

(Vedelek et al., 2016)

testis

(Vedelek et al., 2016)

Detailed Description

Statement

Reference

bb8^MB10362 homozygous as well as bb8^MB10362/Df(3R)Exel9012 and bb8^MB10362/Df(3R)BSC619 hemizygous mutant males (but nor females) are completely sterile. bb8^MB10362 mutant males have empty seminal vesicles without any mature sperm and morphological defects are observed during later stages of spermatogenesis: the spermatid nuclei are elongated correctly but are often scattered over the apical area of the bundles. The bb8^MB10362 mutants have significantly decreased number of elongated spermatids (AXO 49-positive) per testis and these are also significantly shorter compared to wild-type controls. Spermatid individualization is impaired (proper individualization complex fails to be established) and their mitochondria are swollen (megamitochondria). As in wild-type two mitochondrial derivatives are found in bb8^MB10362 mutant spermatids but unlike in wild-type they remain similar to each other in early elongating spermatids and both accumulate paracrystalline, whose structure however is irregular.

(Vedelek et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

bb8^MB10362 has male sterile | recessive phenotype, non-suppressible by wam^SR2/wam^SR2

(Bauerly et al., 2020)

NOT Enhancer of

Statement

Reference

bb8^MB10362 is a non-enhancer of male semi-fertile | dominant phenotype of wam^KO

(Bauerly et al., 2020)

NOT Suppressor of

Statement

Reference

bb8^MB10362 is a non-suppressor of male semi-fertile | dominant phenotype of wam^KO

(Bauerly et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

bb8^MB10362 is rescued by bb8^+t3

(Vedelek et al., 2016)

Comments

The complete male sterility as well as the spermatid individualization defect observed in bb8^MB10362 mutants is fully rescued by combination with bb8^+t3.

(Vedelek et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27841

w¹¹¹⁸; Mi{ET1}bb8^MB10362

Notes on Origin

Discoverer

Comments

Precise excision of the insertion reverts the mutant phenotype.

(Vedelek et al., 2016)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG4434^MB10362

(Vedelek et al., 2016, Bellen, 2009.5.28)

bb8^MB10362

(Bauerly et al., 2020)

bb8^ms

(Vedelek et al., 2023, Vedelek et al., 2016)

Name Synonyms

Secondary FlyBase IDs

FBal0322582
FBal0221424

References (5)

Report Sections

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