FlyBase Allele Report: Dmel\NimB5[SK1]

General Information

Symbol

Dmel\NimB5^SK1

Species

D. melanogaster

Name

FlyBase ID

FBal0362407

Feature type

allele

Associated gene

Dmel\NimB5

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Ramond et al., 2020)

Progenitor genotype

Cytology

Description

13bp deletion in the first exon of NimB5. This generates a premature stop codon, leading to a truncated protein of 54 amino acid residues (instead of the wild-type 351 residues).

(Ramond et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:13,970,716..13,970,729 [+]

Comment:

A 13bp deletion starting in codon 53 leads to a frameshift and early translation termination after the addition of two novel amino acids.

(Ramond et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval crystal cell | larval stage

(Ramond et al., 2020)

embryonic/larval hemocyte | increased number | larval stage (with Df(2L)ED793)

(Ramond et al., 2020)

embryonic/larval hemocyte | increased number | third instar larval stage

(Ramond et al., 2020)

embryonic/larval hemocyte | P-stage | increased number

(Ramond et al., 2020)

lipid droplet | larval stage

(Ramond et al., 2020)

Detailed Description

Statement

Reference

NimB5^SK1 homozygous larvae raised on a normal or a poor diet exhibit an increase in the number hemocytes. NimB5^SK1 homozygous larvae exhibit a higher frequency of mitotic hemocytes and smaller lipid droplets with reduced intensity in fat body.

NimB5^SK1 homozygous third instar larval hemocytes exhibit adhesion defects. On the inner side of the cuticle larvae, hemocytes spread less and lack filopodia. Also hemocytes are only loosely attached to the inner wall of the larvae.

In NimB5^SK1 homozygous larvae the absolute number of crystal cells per larva is higher while the number of melanized crystal cells attached underneath the integument are lower compared to controls. Also the ratio of crystal cells over plasmatocytes is lower.

NimB5^SK1 homozygotes raised on poor diet exhibit partial lethality at pharate adult stage.

The endoparasitoid wasp L. boulardi is less successful to infect NimB5^SK1 homozygotes.

(Ramond et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

NimB5^SK1 has increased mortality during development phenotype, suppressible by Scer\GAL4^Hml.Δ/Mmus\Bax^UAS.cGa

(Ramond et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

NimB5^SK1 is rescued by Scer\GAL4^apolpp.PB/NimB5^UAS.cRa

(Ramond et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

NimB5^SK1

(Ramond et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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