UAS regulatory sequences drive expression of two sgRNAs, both of which target wg. The two sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.
decreased size | adult stage, with Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2
increased cell death | larval stage, with Scer\GAL4hh.PU, Spyo\Cas9UAS.P2
visible | adult stage, with Scer\GAL4hh.PU, Spyo\Cas9UAS.P2
visible | adult stage, with Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2
wing, with Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2
wing disc posterior compartment | larval stage, with Scer\GAL4hh.PU, Spyo\Cas9UAS.P2
wing margin, with Scer\GAL4hh.PU, Spyo\Cas9UAS.P2
wing margin, with Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt4UAS.Wnt4+Wnt6.pCFD6, Wnt6UAS.Wnt4+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has visible | adult stage phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt4UAS.Wnt4+Wnt6.pCFD6, Wnt6UAS.Wnt4+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has decreased size | adult stage phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has visible | adult stage phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has decreased size | adult stage phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt4UAS.Wnt4+Wnt6.pCFD6, Wnt6UAS.Wnt4+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has wing phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt4UAS.Wnt4+Wnt6.pCFD6, Wnt6UAS.Wnt4+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has wing margin phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has wing phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has wing margin phenotype