UAS regulatory sequences drive expression of four sgRNAs; two target Wnt2 and two target Wnt6. The sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has visible | adult stage phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has decreased size | adult stage phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has wing phenotype
Scer\GAL4nub-AC-62, Spyo\Cas9UAS.P2, Wnt2UAS.Wnt2+Wnt6.pCFD6, Wnt6UAS.Wnt2+Wnt6.pCFD6, wgUAS.gRNAx2.pCFD6 has wing margin phenotype