FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ykiS74,97A
Open Close
General Information
Symbol
Dmel\ykiS74,97A
Species
D. melanogaster
Name
FlyBase ID
FBal0363196
Feature type
allele
Associated gene
Dmel\yki
Associated Insertion(s)
TI{TI}ykiS74,97A
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Tyra et al., 2020)
gene targeting by homologous recombination
(Tyra et al., 2020)
Progenitor genotype
Associated Insertion(s)
TI{TI}ykiS74,97A
Cytology
Description

The S74 and 97A mutations are introduced into endogenous yki, along with a 3xTag:FLAG at the N-terminal.

(Tyra et al., 2020)
Allele components
Component
Use(s)
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:24,068,027..24,068,027 [-]
Nucleotide change:

T24068027G

Amino acid change:

S74A | yki-PD; S74A | yki-PF; S74A | yki-PG; S97A | yki-PH

Comment:

Inferred mutation leading to a S74A mutation in a critical Serine. This is one of two Serines mutated in the strain. The other is S97. A 3xTag:FLAG at the N-terminus was also introduced in this strain.

(Tyra et al., 2020)
point_mutation
2R:24,067,958..24,067,958 [-]
Nucleotide change:

T24067958G

Amino acid change:

S97A | yki-PD; S97A | yki-PF; S97A | yki-PG; S120A | yki-PH

Reported amino acid change:

S97A

Comment:

Inferred mutation leading to a S97A mutation in a critical Serine. This is one of two Serines mutated in the strain. The other is S74. A 3xTag:FLAG at the N-terminus was also introduced in this strain.

(Tyra et al., 2020)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ykiS74,97A
      (Tyra et al., 2020)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)