FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Zzzz\scFvC4.UAS.Tag:HA
Open Close
General Information
Symbol
Zzzz\scFvC4.UAS.Tag:HA
Species
a. artificial
Name
FlyBase ID
FBal0363901
Feature type
allele
Associated gene
Zzzz\scFv
Associated Insertion(s)
Carried in Construct
P{UAS-scFv(C4).HA}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wolfgang et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-scFv(C4).HA}
Cytology
Description

UASt regulatory sequences drive expression of the C4 single-chain variable fragment antibody; this targets Hsap\HTT, binding to an epitope formed by the N-terminal 17 amino acids of Hsap\HTT.

(Wolfgang et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Zzzz\scFv
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression with Zzzz\scFvC4.UAS.Tag:HA rescues adult survival of Hsap\HTTQ93.ex1.UAS; Scer\GAL4elav-C155 animals to 100%. The mean and median survival time for Hsap\HTTQ93.ex1.UAS adults co-expressing Zzzz\scFvC4.UAS.Tag:HA, under the control of Scer\GAL4elav-C155, compared to flies expressing solely Hsap\HTTQ93.ex1.UAS is increased significantly, with increases ranging from 30% to 50%. Maximum lifespan is also significantly improved, although not to that of wild-type levels. Genetically doubling the dose of Zzzz\scFvC4.UAS.Tag:HA produces no further improvement in survival.

      Co-expression of Zzzz\scFvC4.UAS.Tag:HA partially suppresses the age-dependent loss of photoreceptor cells seen in Hsap\HTTQ93.ex1.UAS; Scer\GAL4elav-C155 flies.

      (Wolfgang et al., 2005)
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/Zzzz\scFvC4.UAS.Tag:HA rescues Hsap\HTTQ93.ex1.UAS

      (Bortvedt et al., 2010)
      Comments

      In Hsap\HDQ93.ex1p.Scer\UAS-expressing flies expressing the Hsap\HDC4sFv.Scer\UAS.T:Ivir\HA1 transgene, both under the control of Scer\GAL4elav-C155, photoreceptors are significantly rescued by 48% compared to no intrabody controls.

      Co-expression of Hsap\HDC4sFv.Scer\UAS.T:Ivir\HA1 confers significantly improved survival on Hsap\HDQ93.ex1p.Scer\UAS flies.

      (Bortvedt et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Hsap\HDC4sFv.Scer\UAS.T:Ivir\HA1
      Hsap\HTTC4sFv.Scer\UAS.T:Ivir\HA1
      Hsap\HTTC4sFv.UAS.Tag:HA
      Zzzz\scFvC4.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0363668
      • FBal0190959
      References (4)