FlyBase Allele Report: Spyo\Cas9[dxCas9-3.7::VPR.αTub84B]

General Information

Symbol

Spyo\Cas9^{dxCas9-3.7::VPR.αTub84B}

Species

S. pyogenes

Name

FlyBase ID

FBal0363984

Feature type

allele

Associated gene

Spyo\Cas9

Associated Insertion(s)

Carried in Construct

PBac{αTub84B-dxCas9-3.7::VPR.M}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Maselko et al., 2020)

Progenitor genotype

Carried in construct

PBac{αTub84B-dxCas9-3.7::VPR.M}

Cytology

Description

A short αTub84B promoter drives expression of the dxCas9-3.7::VPR transcriptional activator.

(Maselko et al., 2020)

Allele components

Component

Use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

dxCas9-3.7::VPR

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | female, with upd2^TOE.GS00171

(Maselko et al., 2020)

lethal - all die before end of P-stage, with pyr^TOE.GS00085

(Maselko et al., 2020)

lethal - all die before end of P-stage, with wg^gRNAx2

(Maselko et al., 2020)

partially lethal - majority die | female, with upd1^TOE.GS00169

(Maselko et al., 2020)

viable, with hh^TOE.GS00191

(Maselko et al., 2020)

viable, with hid^{U6.gRNAx2.pCFD4}

(Maselko et al., 2020)

viable, with upd3^TOE.GS00129

(Maselko et al., 2020)

viable, with vn^TOE.GS00144

(Maselko et al., 2020)

viable | male, with upd1^TOE.GS00169

(Maselko et al., 2020)

viable | male, with upd2^TOE.GS00171

(Maselko et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93595

y¹ w¹¹¹⁸; PBac{αTub84B-dxCas9-3.7::VPR.M}VK00027

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Spyo\Cas9^{dxCas9-3.7::VPR.αTub84B}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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