dxCas9-3.7::VPR is an artificial transcriptional activator ('driver') that contains a nuclease-dead form of the Streptococcus pyogenes Cas9 gene (contains the mutations D10A, H839A, H840A and N863A) fused to a tripartite fusion of three transcription activation domains: VP64, p65 and Rta. It can be used to overexpress a target gene of interest from its endogenous genomic locus, by combining it with a single guide RNA (sgRNA) that targets sequences upstream of the gene of interest's transcription start site. The Cas9 sequence in dxCas9-3.7::VPR contains a number of additional mutations compared to the wild-type Streptococcus pyogenes Cas9 gene (A262T, R324L, S409I, E480K, E543D, M694I and E1219V); these result in the dxCas9-3.7::VPR driver having a broadened protospacer adjacent motif (PAM) compatibility compared to the dCas9::VPR driver (PMID:29512652, FBrf0246662).