FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Experimental tool: dCas9::p300(core)
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General Information
Symbol
dCas9::p300(core)
FlyBase ID
FBto0000934
Name
Description
Description

dCas9::p300(core) is an artificial transcriptional activator that contains a nuclease-dead form of the Streptococcus pyogenes Cas9 gene (contains the amino acid substitutions D10A and H840A) fused to a fragment corresponding to the catalytic core (codons 1048-1664, which includes the bromodomain, RING domain, PHD domain and histone acetyltransferase domain, see PMID:23934153) of the histone acetyltransferase encoded by the Homo sapiens EP300 gene (HGNC:3373). This driver can be used to overexpress a target gene of interest from its endogenous genomic locus, by combining it with a single guide RNA (sgRNA) that targets sequences upstream of the gene of interest's transcription start site. The driver is capable of activating transcription from both promoter proximal and distal enhancer regions (PMID:25849900).

External Crossreferences and Linkouts
Related experimental tools
Transgenic Constructs
Encodes tool (1)
Insertions ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
dCas9::p300(core)
dCas9<up>p300 Core</up>
Name Synonyms
Secondary FlyBase IDs
    References (2)