dCBE(evoCDA1) is an artificial 'base editor' that can be used to irreversibly convert a single target cytosine DNA base in a programmable manner, without requiring double-stranded cleavage of the DNA backbone or a donor template; it favors the conversion of C to T. dCBE(evoCDA1) is a fusion protein composed of 1. a laboratory evolved version of the cytidine deaminase 1 (CDA1) protein from Petromyzon marinus, 2. the Cas9(D10A) nickase and 3. a uracil DNA glycosylase inhibitor (UGI). Each coding sequence is fused via a linker, and bipartite nuclear localization signals are present at the N-terminal and C-terminal ends of the fusion protein; these sequences are based on the optimized 'BE4max' architecture from PMID:29813047 (FBrf0257420).