FB2026_03 , released September 17, 2026
Experimental tool: Cas9(H840A)
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General Information
Symbol
Cas9(H840A)
FlyBase ID
FBto0001099
Name
Cas9(H840A) nickase
Description
Description

The Cas9(H840A) entry in FlyBase represents a RNA-guided nuclease that is derived from the naturally occurring Cas9 endonuclease from Streptococcus pyogenes and which contains an inactivating point mutation (H840A) in the HNH endonuclease domain (the RuvC endonuclease domain is not mutated). This converts the endonuclease from one that generates double stranded breaks in DNA to a 'nickase', which cleaves only the non-target strand containing the protospacer adjacent motif sequence when targeted to a genomic target site of interest using a guide RNA (gRNA).

Uses
External Crossreferences and Linkouts
Related experimental tools
Common gene of origin (30)
Tool
Uses
BE3
Cas9
Cas9::CMP(Unk)
Cas9::HN1HB1
Cas9(D10A)
Cas9(R691A)
DBE2
dCas9::GS
dCas9::GSD
dCas9::nej(core)
dCas9::p300(core)
dCas9::VP64
dCas9::VP64b
dCas9::VPR
dCas9(D10A.H840A)
dCas9(D10A.N863A)
dCBE(ancBE4::Cas9(D10A)::NLS)
dCBE(evoAPOBEC1)
dCBE(evoCDA1)
dCBE(hAICDA(starĪ”)::Cas9(D10A)::NLS)
dCBE(rAPOBEC1::Cas9(D10A)::NLS)
dxCas9-3.7::VPR
eSpCas9(1.1)
eyDBD::Cas9
FKBP::C-Cas9(5)
FKBP::C-dCas9(4)::VPR
FokI::dCas9
N-Cas9(5)::FRB
N-dCas9(4)::FRB
PE2
Transgenic Constructs
Encodes tool (2)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Spyo\Cas9H840A.zk
Cas9(H840A)
Tag:NLS(Unk)
Spyo\Cas9H840A.vas
Cas9(H840A)
Insertions ( 2 )
Insertion
Encoded product / tool
Tagged with
Also carries
Stocks
Associated Alleles
P{Cas9.H840A.G4HACK}attP40
Cas9(H840A)
    P{ET-Cas9.H840A.GU}hhCas9.H840A
    Cas9(H840A)
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Cas9(H840A)
    Name Synonyms
    Cas9(H840A) nickase
    Secondary FlyBase IDs
      References (4)