A TI{CRIMIC.TG4.1} DNA cassette has been inserted into CG31371, in a coding intron, and is predicted to gene trap 1 of the 2 annotated transcripts of CG31371; the intron in the other transcript is out of phase. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CATTCTCACAGTCATTTCTTTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 22bp of genomic sequence flanking the insertion site.