2385bp of robo1 upstream flanking sequence drives expression of a deleted form of robo1 that lacks 4 of the 5 immunoglobulin-like domains (Ig2-5 are deleted). 2192bp of robo1 downstream flanking sequence is also present. The protein is tagged directly upstream of the Ig1 domain with 4 copies of Tag:HA.
Scer\GAL4elav.PLu, robo1ΔIg2-5.Tag:HA, robo11, robo2UAS.Tag:HA,Tag:SS(wg) has axon | embryonic stage phenotype
Scer\GAL4elav.PLu, commUAS.cUa, robo1ΔIg2-5.Tag:HA has axon | embryonic stage phenotype