FlyBase Allele Report: Spyo\Cas9[repo.PK]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Spyo\Cas9^repo.PK

General Information

Symbol

Spyo\Cas9^repo.PK

Species

S. pyogenes

Name

repo promoter construct of Koreman

FlyBase ID

FBal0368074

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

repo-Cas9

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Koreman et al., 2021)

Progenitor genotype

Carried in construct

Cytology

Description

A 4176 bp repo enhancer regulates expression of Spyo\Cas9.

(Koreman et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

RNA-guided nuclease

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Nsf2^{U6:3.gRNA.GGGG(FE)}, Spyo\Cas9^repo.PK, comt^{U6:3.gRNA.GGGG(FE)} has lethal - all die before end of larval stage phenotype

(Chen et al., 2024)

Nsf2^{U6:3.gRNA.GGGG(FE)}, Spyo\Cas9^repo.PK, comt^{U6:3.gRNA.GGGG(FE)} has decreased rate of larval locomotory behavior phenotype

(Chen et al., 2024)

Phenotype Manifest In

Other

Statement

Reference

Nsf2^{U6:3.gRNA.GGGG(FE)}, Spyo\Cas9^repo.PK, comt^{U6:3.gRNA.GGGG(FE)} has larval glial cell phenotype

(Chen et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

92760

w^* P{repo-Cas9.K}5D

92761

w^*; P{repo-Cas9.K}6A/CyO, P{Wee-P.ph0}2; TM2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Spyo\Cas9^repo.PK

Name Synonyms

repo promoter construct of Koreman

Secondary FlyBase IDs

References (4)

Report Sections