FlyBase Allele Report: Spyo\Cas9[VGlut1-OK371]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Spyo\Cas9^VGlut1-OK371

General Information

Symbol

Spyo\Cas9^VGlut1-OK371

Species

S. pyogenes

Name

FlyBase ID

FBal0368075

Feature type

allele

Associated gene

Associated Insertion(s)

P{ET-Cas9.GB}VGlut1^OK371

Carried in Construct

Also Known As

OK371-Cas9

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

gene conversion

(Koreman et al., 2021)

(Koreman et al., 2021)

Progenitor genotype

P{GawB}VGlut1^OK371

(Koreman et al., 2021)

Associated Insertion(s)

P{ET-Cas9.GB}VGlut1^OK371

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

RNA-guided nuclease

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | larval stage, with Syt1^U6.gRNAx3

(Chen et al., 2024)

Phenotype Manifest In

extracellular vesicle | decreased number | larval stage, with shrb^U6.gRNAx2

(Chen et al., 2024)

extracellular vesicle | decreased number | larval stage, with TSG101^U6.gRNAx2

(Chen et al., 2024)

hypodermal muscle of A1-7 | larval stage, with Syt1^U6.gRNAx3

(Chen et al., 2024)

intracellular vesicle | larval stage, with shrb^U6.gRNAx2

(Chen et al., 2024)

intracellular vesicle | larval stage, with TSG101^U6.gRNAx2

(Chen et al., 2024)

NMJ bouton | decreased number | larval stage, with Syx5^U6:3.gRNA.GQ

(Chen et al., 2024)

NMJ bouton | increased number | larval stage, with shrb^U6.gRNAx2

(Chen et al., 2024)

NMJ bouton | increased number | larval stage, with TSG101^U6.gRNAx2

(Chen et al., 2024)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Snap24^{U6:3.gRNA.Snap24-Snap25-Snap29}, Snap25^{U6:3.gRNA.Snap24-Snap25-Snap29}, Snap29^{U6:3.gRNA.Snap24-Snap25-Snap29}, Spyo\Cas9^VGlut1-OK371 has NMJ bouton | larval stage | decreased number phenotype

(Chen et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

92752

w^*; P{ET-Cas9.GB}VGlut1^OK371/CyO, P{Wee-P.ph0}Bacc^Wee-P20; TM2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Spyo\Cas9^VGlut-OK371

Spyo\Cas9^VGlut1-OK371

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections