FlyBase Insertion Report: Dmel\P{GawB}VGlut[OK371]

General Information

Symbol

Dmel\P{GawB}VGlut^OK371

Species

D. melanogaster

Name

FlyBase ID

FBti0076967

Feature type

transposable_element_insertion_site

Also Known As

OK371-GAL4, Vglut-Gal4, OK371, vGlut^OK371-Gal4, OK371Gal4, GAL4-OK371, OK371 GAL4

Description

Inserted Element

P{GawB}

Uses

GAL4 reporter/driver

Affected gene(s)

Scer\GAL4, VGlut

Viability / fertility

viable

Associated allele(s)

Scer\GAL4^VGlut-OK371, VGlut^OK371

Stock availability

3 publicly available

LINE ID

Genomic Location

Chromosomal Location

2L ( 22E1 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

22E1 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Mahr and Aberle, 2006)

Location-dependent role

Size (Kb)

11.279

Associated alleles

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Heinrich and Ryglewski, 2020, Beck et al., 2012, Dickman et al., 2012, Jepson et al., 2012, Jochum et al., 2012, Lloyd et al., 2012, Lotti et al., 2012, Majumdar et al., 2012, Oyallon et al., 2012, Panz et al., 2012, Xia et al., 2012, Abou Tayoun et al., 2011, Cheng et al., 2011, Cognigni et al., 2011, Jepson et al., 2011, Keegan et al., 2011, Keller et al., 2011, Lanson et al., 2011, Shao et al., 2011, Slawson et al., 2011, Boerner and Duch, 2010, de Haro et al., 2010, Li et al., 2010, Li et al., 2010, Melicharek et al., 2010, Nedelsky et al., 2010, Nicolaï et al., 2010, Yu et al., 2010, Baek and Mann, 2009, Hornstein et al., 2009, Jansen et al., 2009, Johnson et al., 2009, Pulver et al., 2009, Venkiteswaran and Hasan, 2009, Diegelmann et al., 2008, Gao et al., 2008, Gao et al., 2008, Grosjean et al., 2008, Koch et al., 2008, Kolodziejczyk et al., 2008, Nässel et al., 2008, Beuchle et al., 2007, Mahr and Aberle, 2006, Meyer and Aberle, 2006)

VGlut

(Baek and Mann, 2009, Mahr and Aberle, 2006)

Alleles and Phenotypes

Allele

Scer\GAL4^VGlut-OK371

Reference(s)

(Baek et al., 2021, Hsu et al., 2021, Koreman et al., 2021, Sidisky et al., 2021, Song et al., 2021, Wong et al., 2021, Azevedo et al., 2020, Cunningham et al., 2020, Genç et al., 2020, Gunderson et al., 2020, Hakes and Brand, 2020, Heinrich and Ryglewski, 2020, Kankel et al., 2020, Ma et al., 2020, Nandakumar et al., 2020, Odierna et al., 2020, Pop et al., 2020, Rallis et al., 2020, Ramesh et al., 2020, Spinner et al., 2020, Wang et al., 2020, Wang et al., 2020, Wen et al., 2020, Yalgin et al., 2020, Zhang et al., 2020, Casci et al., 2019, Chen et al., 2019, Connolly et al., 2019, De Graeve et al., 2019, Gratz et al., 2019, Held et al., 2019, Hunt et al., 2019, Kaur et al., 2019, Kulik et al., 2019, Lowe et al., 2019, Moss-Taylor et al., 2019, Peng et al., 2019, Schützler et al., 2019, Shepherd et al., 2019, Smith et al., 2019, Takayanagi-Kiya and Kiya, 2019, Trostnikov et al., 2019, Wang et al., 2019, Yuva-Aydemir et al., 2019, Badders et al., 2018, Constance et al., 2018, Davie et al., 2018, Dean et al., 2018, Deng et al., 2018, El Fissi et al., 2018, Enriquez et al., 2018, Farley et al., 2018, Gowda et al., 2018, Harris et al., 2018, Hauswirth et al., 2018, Kinold et al., 2018, Liao et al., 2018, Mallik et al., 2018, Robinson et al., 2018, Sonn et al., 2018, Xu and Xu, 2018, Yan et al., 2018, Akbergenova and Littleton, 2017, Celona et al., 2017, Fernius et al., 2017, Genç et al., 2017, Horne et al., 2017, Jeong et al., 2017, King et al., 2017, Kiragasi et al., 2017, Larderet et al., 2017, Li et al., 2017, Mauss et al., 2017, Neukomm et al., 2017, Orr et al., 2017, Pathak et al., 2017, Reddy-Alla et al., 2017, Schwarz et al., 2017, Smart et al., 2017, Solis et al., 2017, Wietek et al., 2017, Wu et al., 2017, Yoon et al., 2017, Banerjee et al., 2016, Clark et al., 2016, Colomb and Brembs, 2016, D'Rozario et al., 2016, Friedrich et al., 2016, Lee et al., 2016, Li et al., 2016, Shepherd et al., 2016, Spring et al., 2016, Summerville et al., 2016, Syed et al., 2016, Tio et al., 2016, Vagnoni and Bullock, 2016, Yilmazer et al., 2016, Ziegler et al., 2016, Agrawal and Hasan, 2015, Cagin et al., 2015, Deng et al., 2015, Diao et al., 2015, Erdmann et al., 2015, Freibaum et al., 2015, Harris et al., 2015, Jäckel et al., 2015, Jeong et al., 2015, Matzat et al., 2015, Niehues et al., 2015, Okamoto and Nishimura, 2015, Pathak et al., 2015, Sreedharan et al., 2015, Stephan et al., 2015, Tran et al., 2015, Wong et al., 2015, Yang et al., 2015, Zhang et al., 2015, Bath et al., 2014, Bulat et al., 2014, Chak and Kolodkin, 2014, Kemppainen et al., 2014, Klapoetke et al., 2014, Koch et al., 2014, Kunst et al., 2014, Lüchtenborg and Katanaev, 2014, Lüchtenborg et al., 2014, Machamer et al., 2014, Nakayama et al., 2014, Neukomm et al., 2014, Roy and Jackson, 2014, Schoofs et al., 2014, Stork et al., 2014, Takayama et al., 2014, Wen et al., 2014, Wong et al., 2014, Zhang et al., 2014, Akerboom et al., 2013, Castellanos et al., 2013, Daigle et al., 2013, Das et al., 2013, Enneking et al., 2013, Gillespie and Hodge, 2013, Kim et al., 2013, Li et al., 2013, Ni et al., 2013, Shahidullah et al., 2013, Xu et al., 2013, Younger et al., 2013, Zhu et al., 2013, Beck et al., 2012, Brierley et al., 2012, Cavaliere et al., 2012, Dickman et al., 2012, Holbrook et al., 2012, Imlach et al., 2012, Jepson et al., 2012, Jochum et al., 2012, Lloyd et al., 2012, Lotti et al., 2012, Majumdar et al., 2012, Oyallon et al., 2012, Panz et al., 2012, Smith et al., 2012, Timmerman and Sanyal, 2012, Wang et al., 2012, Xia et al., 2012, Zhou et al., 2012, Abou Tayoun et al., 2011, Chen et al., 2011, Cheng et al., 2011, Cognigni et al., 2011, Das et al., 2011, Demilly et al., 2011, Guo et al., 2011, Jepson et al., 2011, Keegan et al., 2011, Keller et al., 2011, Lanson et al., 2011, Pielage et al., 2011, Shao et al., 2011, Slawson et al., 2011, Boerner and Duch, 2010, de Haro et al., 2010, Li et al., 2010, Li et al., 2010, Melicharek et al., 2010, Nedelsky et al., 2010, Nicolaï et al., 2010, Yu et al., 2010, Baek and Mann, 2009, Brierley et al., 2009, Hornstein et al., 2009, Jansen et al., 2009, Johnson et al., 2009, Pulver et al., 2009, Venkiteswaran and Hasan, 2009, Diegelmann et al., 2008, Gao et al., 2008, Gao et al., 2008, Grosjean et al., 2008, Johard et al., 2008, Koch et al., 2008, Kolodziejczyk et al., 2008, Miguel-Aliaga et al., 2008, Nässel et al., 2008, Beuchle et al., 2007, Mahr and Aberle, 2006, Meyer and Aberle, 2006)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

VGlut^OK371

Reference(s)

(Baek and Mann, 2009)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval protocerebrum | restricted

(Nässel et al., 2008)

adult mushroom body beta-lobe | surrounding

(Nässel et al., 2008)

larval ventral nerve cord

(Nässel et al., 2008)

third instar larval stage

glutamatergic neuron

(de Haro et al., 2010)

medial lobe of larval mushroom body

(Johard et al., 2008)

spur of larval mushroom body

(Johard et al., 2008)

embryonic/larval mushroom body

(Johard et al., 2008)

larval prothoracic neuromere of central nervous system

(Brierley et al., 2012)

third instar larval stage -- P-stage

motor neuron of prothoracic leg

(Brierley et al., 2012)

Additional Information

Statement

Reference

Drives expression in glutamatergic neurons. Drives adult expression in neurites of mushroom body extrinsic neurons in the heel, and especially in the medial part of the beta and gamma lobes. Drives larval expression in a similar pattern, with additional expression around the peduncle and vertical lobe, as well as in the protocerebrum and ventral nerve cord.

(FlyBase Curators, 2017)

Positive GRASP (GFP reconstitution across synaptic partners) signals between Scer\GAL4^VGlut-OK371 and adult Hugin neurons labelled with Ecol\lexA^GMR61H10 were observed in the thoracic and abdominal ganglia.

(King et al., 2017)

Expression of Scer\GAL4^VGlut-OK371, which reflects expression of VGlut, within the protocerebrum, is seen within a dorso-laterally located group of cells, and where it colocalizes with sNPF protein. Expression of Scer\GAL4^VGlut-OK371 in the nerve cord is extensive.

(Nässel et al., 2008)

In adults, Scer\GAL4^VGlut-OK371 drives expression in neurites of mushroom body extrinsic neurons in the heel, and especially in the medial part of the beta and gamma lobes. Larval expression was similar to the one in adults, with additional expression around the peduncle and vertical lobe.

(Johard et al., 2008)

Marker for

glutamatergic neuron

(Kolodziejczyk et al., 2008)

Reflects expression of

VGlut

(King et al., 2017, de Haro et al., 2010, Yu et al., 2010, Gao et al., 2008, Johard et al., 2008, Kolodziejczyk et al., 2008)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(de Haro et al., 2010, Johard et al., 2008, Kolodziejczyk et al., 2008, Nässel et al., 2008)

Mmus\Cd8a^{FRT.UAS.cYa.GFP}

(Yu et al., 2010)

nSyb^UAS.EGFP

(Kolodziejczyk et al., 2008)

Nrx-1^{UAS.spGFP1-10}

(King et al., 2017)

Avic\GFP^{spGFP11.lexAop.Tag:M(hCD4)}

(King et al., 2017)

Ecol\lexA^GMR61H10

(King et al., 2017)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (3)

Bloomington

26160

w¹¹¹⁸; P{GawB}VGlut^OK371

79601

w¹¹¹⁸; P{GawB}VGlut^OK371, P{UAS-H134R-ChR2}2; Snap25^ts

79600

w¹¹¹⁸; P{GawB}VGlut^OK371, P{UAS-H134R-ChR2}2, P{Mhc-GFP.F4-453}2/CyO

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (41)

Reported As

Symbol Synonym

GAL4-OK371

(Das et al., 2013)

Gal4-OK371

(Cavaliere et al., 2012, Das et al., 2011)

GawB V Glut^OK371

(Wang et al., 2020)

Glutamatergic GAL4

(Venkiteswaran and Hasan, 2009)

OK371

(Yalgin et al., 2020, Chen et al., 2019, Shepherd et al., 2019, Takayanagi-Kiya and Kiya, 2019, Colomb and Brembs, 2016, Shepherd et al., 2016, Pathak et al., 2015, Stephan et al., 2015, Kemppainen et al., 2014, Stork et al., 2014, Majumdar et al., 2012, Keegan et al., 2011, Yu et al., 2010, Venkiteswaran and Hasan, 2009)

OK371 GAL4

(Wen et al., 2014)

OK371 Gal4

(Smith et al., 2012)

OK371-G4

(Kim et al., 2013)

OK371-GAL4

(Baek et al., 2021, Azevedo et al., 2020, Gunderson et al., 2020, Hakes and Brand, 2020, Kankel et al., 2020, Ma et al., 2020, Nandakumar et al., 2020, Odierna et al., 2020, Wen et al., 2020, Kulik et al., 2019, Peng et al., 2019, Schützler et al., 2019, Badders et al., 2018, Constance et al., 2018, El Fissi et al., 2018, Farley et al., 2018, Hauswirth et al., 2018, Mallik et al., 2018, Akbergenova and Littleton, 2017, Celona et al., 2017, Horne et al., 2017, Jeong et al., 2017, Larderet et al., 2017, Orr et al., 2017, Smart et al., 2017, Wu et al., 2017, Yoon et al., 2017, Friedrich et al., 2016, Spring et al., 2016, Tio et al., 2016, Freibaum et al., 2015, Jäckel et al., 2015, Niehues et al., 2015, Zhang et al., 2015, Bath et al., 2014, Machamer et al., 2014, Akerboom et al., 2013, Castellanos et al., 2013, Kim et al., 2013, Ni et al., 2013, Shahidullah et al., 2013, Younger et al., 2013, Zhu et al., 2013, Brierley et al., 2012, Dickman et al., 2012, Jochum et al., 2012, Lloyd et al., 2012, Timmerman and Sanyal, 2012, Zhou et al., 2012, Keller et al., 2011, Lanson et al., 2011, Boerner and Duch, 2010, de Haro et al., 2010, Nedelsky et al., 2010, Nicolaï et al., 2010, Brierley et al., 2009, Hornstein et al., 2009, Jansen et al., 2009, Johnson et al., 2009, Pulver et al., 2009, Miguel-Aliaga et al., 2008)

OK371-GAl4

(Kunst et al., 2014)

OK371-Gal4

(Hsu et al., 2021, Koreman et al., 2021, Sidisky et al., 2021, Pop et al., 2020, Ramesh et al., 2020, Spinner et al., 2020, Wang et al., 2020, Zhang et al., 2020, Connolly et al., 2019, De Graeve et al., 2019, Gratz et al., 2019, Held et al., 2019, Hunt et al., 2019, Kaur et al., 2019, Lowe et al., 2019, Moss-Taylor et al., 2019, Smith et al., 2019, Wang et al., 2019, Yuva-Aydemir et al., 2019, Davie et al., 2018, Deng et al., 2018, Enriquez et al., 2018, Gowda et al., 2018, Kinold et al., 2018, Liao et al., 2018, Robinson et al., 2018, Sonn et al., 2018, Fernius et al., 2017, Genç et al., 2017, Kiragasi et al., 2017, Li et al., 2017, Neukomm et al., 2017, Schwarz et al., 2017, Solis et al., 2017, Banerjee et al., 2016, Clark et al., 2016, D'Rozario et al., 2016, Lee et al., 2016, Summerville et al., 2016, Syed et al., 2016, Ziegler et al., 2016, Cagin et al., 2015, Deng et al., 2015, Diao et al., 2015, Erdmann et al., 2015, Harris et al., 2015, Matzat et al., 2015, Sreedharan et al., 2015, Yang et al., 2015, Klapoetke et al., 2014, Koch et al., 2014, Lüchtenborg and Katanaev, 2014, Lüchtenborg et al., 2014, Nakayama et al., 2014, Neukomm et al., 2014, Schoofs et al., 2014, Takayama et al., 2014, Gillespie and Hodge, 2013, Beck et al., 2012, Holbrook et al., 2012, Imlach et al., 2012, Jepson et al., 2012, Lotti et al., 2012, Oyallon et al., 2012, Panz et al., 2012, Wang et al., 2012, Xia et al., 2012, Abou Tayoun et al., 2011, Chen et al., 2011, Cheng et al., 2011, Cognigni et al., 2011, Guo et al., 2011, Slawson et al., 2011, Li et al., 2010, Melicharek et al., 2010, Baek and Mann, 2009, Diegelmann et al., 2008, Gao et al., 2008, Johard et al., 2008, Koch et al., 2008, Kolodziejczyk et al., 2008, Nässel et al., 2008, Beuchle et al., 2007, Mahr and Aberle, 2006, Meyer and Aberle, 2006)

OK371-gal4

(Casci et al., 2019, Daigle et al., 2013)

OK371>GAL4

(Li et al., 2016)

OK371GAL4

(Pathak et al., 2017, Agrawal and Hasan, 2015)

OK371Gal4

(Gowda et al., 2018, Grosjean et al., 2008)

OK³⁷¹-Gal4

(Chak and Kolodkin, 2014)

Ok371

(Jepson et al., 2011)

Ok371-GAL4

(Reddy-Alla et al., 2017, Xu et al., 2013)

Ok371-Gal4

(Harris et al., 2018)

P{GawB}OK371

(Johnson et al., 2009, FlyBase, 1992-)

P{GawB}VGlut^OK371

P{w^mW.hsGawB}vGlut^OK371

(Xu and Xu, 2018)

VGLUT-GAL4

(Roy and Jackson, 2014)

VGLUT^OK371-GAL4

(Roy and Jackson, 2014)

VGlut-GAL4

(Dean et al., 2018, Timmerman and Sanyal, 2012, de Haro et al., 2010)

VGlut-Gal4

(Enriquez et al., 2018, Okamoto and Nishimura, 2015, Shao et al., 2011)

Vglut-Gal4

(Song et al., 2021, Yan et al., 2018, Baek and Mann, 2009)

Vglut^OK371-GAL4

(Yilmazer et al., 2016)

ok371-GAL4

(Wong et al., 2015, Wong et al., 2014, Li et al., 2010)

ok371-Gal4

(Yan et al., 2018, Wietek et al., 2017, Vagnoni and Bullock, 2016, Bulat et al., 2014, Enneking et al., 2013, Li et al., 2013)

ok371-gal4

(Genç et al., 2020)

ok371Gal4

(Pielage et al., 2011)

vGluT-Gal4

(Kolodziejczyk et al., 2008)

vGlut-Gal4

(Cunningham et al., 2020, Rallis et al., 2020, Chen et al., 2019, Connolly et al., 2019, Xu and Xu, 2018, Mauss et al., 2017, Jeong et al., 2015, Zhang et al., 2014, Gao et al., 2008)

vGlut-OK371-Gal4

(Mauss et al., 2017)

vGlut^OK371-GAL4

(Heinrich and Ryglewski, 2020)

vGlut^OK371-Gal4

(Gao et al., 2008)

vesciular glutamate transporter-galactosidase-4

(Baek and Mann, 2009)

vesicular glutamate transporter Gal4

(Shao et al., 2011)

vglut-GAL4

(King et al., 2017)

vglut^ok371-GAL4

(Wong et al., 2021, Wong et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (210)

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