FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ipo9KO
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General Information
Symbol
Dmel\Ipo9KO
Species
D. melanogaster
Name
FlyBase ID
FBal0368297
Feature type
allele
Associated gene
Dmel\Ipo9
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Palacios et al., 2021)
gene targeting by homologous recombination
(Palacios et al., 2021)
Progenitor genotype
Cytology
Description

A sequence cassette consisting of 1. an attP site, and 2. a loxP cassette containing the Disc\RFPDsRed(T1).3xP3.cHa marker has been inserted into the endogenous Ipo9 locus, replacing almost all the transcription unit.

(Palacios et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos from Ipo9KO/Ipo9KO mothers show widespread mitotic defects during the earliest embryonic divisions (chromosome bridges, chromosome fragmentation, lack of chromosome condensation and an array of spindle defects).

      Ipo9KO/Ipo9KO leads to meiotic defects during oogenesis: during stage 14 of oogenesis chromosomes are misoriented/mislocalized.

      Ipo9KO/Ipo9KO leads to defects during spermatogenesis: chromosome segregation defects are apparent; the clustered post-meiotic nuclei remain round well beyond the stage when nuclear shape occurs; spermatids do not go through individualization (no actin cones or waste bags) and mature sperm is absent;at the onion stage there are variably sized nuclei and nebenkern.

      (Palacios et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Ipo9KO is partially rescued by Scer\GAL4vas.PU/Ipo9UASp.Tag:HA

      (Palacios et al., 2021)
      Not rescued by

      Ipo9KO is not rescued by Ipo9ΔN.UASp.Tag:HA/Scer\GAL4vas.PU

      (Palacios et al., 2021)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ipo9KO
      (Palacios et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)