A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Pex23, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. In addition, the insertion is 93 bp upstream of the 5' end of both the SCCRO4 and asRNA:CR43872 genes and may affect the expression of either. The TI{CRIMIC.TG4.2} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: ATTGTTCAATTTATCCAAGCAGG.