A 3xHA-T2A-DsRed2-NLS tag cassette followed by a female stop TGA has been inserted at 3R:7935564..7935565 (release 6 coordinates), tagging the female isoform of the endogenous dsx locus. The 3xHA tag is expected to be incorporated into the female dsx isoform, while the T2A sequence promotes the separate translation of the DsRed2-Tag:NLS(Unk) reporter.
A 3xHA-T2A-DsRed2-NLS tag cassette followed by a female stop TGA has been inserted at 3R:7935564..7935565 (release 6 coordinates).