FlyBase Allele Report: Dmel\NimB4[sk2]

General Information

Symbol

Dmel\NimB4^sk2

Species

D. melanogaster

Name

FlyBase ID

FBal0371440

Feature type

allele

Associated gene

Dmel\NimB4

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Petrignani et al., 2021)

Progenitor genotype

Cytology

Description

14bp deletion in the first exon of NimB4, resulting in a premature stop codon at codon 113. Also carries a G to C nucleotide substitution immediately upstream of the deletion.

(Petrignani et al., 2021)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:13,969,652..13,969,666 [-]

Inserted_sequence:

Comment:

Net deletion of 14 bases and insertion of a C leads to a frameshift and early translation termination.

(Petrignani et al., 2021)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Petrignani et al., 2021)

abnormal locomotor behavior | third instar larval stage

(Petrignani et al., 2021)

increased cell death | embryonic stage

(Petrignani et al., 2021)

increased cell death | larval stage

(Dolgikh et al., 2025, Petrignani et al., 2021)

short lived

(Petrignani et al., 2021)

Phenotype Manifest In

cytoplasmic vesicle | increased number | larval stage

(Petrignani et al., 2021)

embryonic/larval plasmatocyte | embryonic stage

(Petrignani et al., 2021)

embryonic/larval plasmatocyte | larval stage

(Dolgikh et al., 2025, Petrignani et al., 2021)

larval brain | larval stage

(Petrignani et al., 2021)

phagocytic vesicle | increased number | larval stage

(Petrignani et al., 2021)

Detailed Description

Statement

Reference

NimB4^sk2 homozygous embryos exhibit increased apoptosis, and macrophages appear highly vacuolated and full of apoptotic corpses. Larvae also exhibit increased apoptosis in the brain. Larval macrophages exhibit decreased phagocytosis of apoptotic cells but retain the ability to phagocytose S. aureus bacteria; these macrophages also exhibit increased number of large intracellular vesicles, namely phagosomes.

NimB4^sk2 homozygous third instar larvae exhibit decreased crawling ability, namely a severe decrease in linear movement, and adults show decreased climbing ability,

(Petrignani et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

NimB4^sk2 has increased cell death | embryonic stage phenotype, suppressible by Df(3L)H99

(Petrignani et al., 2021)

Other