Deletion producing a frameshift followed by a premature stop codon, resulting in expression of a truncated Amnionless protein lacking the FXNPXF domain required for ligand-independent internalisation, but retaining the three-faced β-helix and the transmembrane domains required in vertebrates for docking of cubilin trimers and their traffic to the membrane (384 wild type amino acids, followed by 6 novel amino acids).
A 7bp deletion in Amnionless starting in codon L386 that causes a frameshift and leads to early translation termination after the addition of 6 novel amino acids.
AmnionlessNSI-1 homozygous larval nephrocytes exhibit aberrant distribution of slit diaphragms in internal membranes, as well as acentric nuclei.