FlyBase Gene Report: Dmel\Amnionless

General Information

Symbol

Dmel\Amnionless

Species

D. melanogaster

Name

Amnionless

Annotation Symbol

CG11592

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025686

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

21B7-21B7

Sequence location

2L:287,252..289,144 [-]

Recombination map

2-0.5

RefSeq locus

NT_033779 REGION:287252..289144

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in nephrocyte filtration

inferred from mutant phenotype

(Zhang et al., 2013)

involved_in renal protein absorption

inferred from mutant phenotype

(Zhang et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein localization

inferred from biological aspect of ancestor with PANTHER:PTN001029969

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in receptor-mediated endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN001029969

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in apical plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001029969

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in endocytic vesicle

inferred from biological aspect of ancestor with PANTHER:PTN001029969

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in integral component of membrane

inferred from sequence model

(Zhang et al., 2013)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Amnionless

Summaries

Protein Function (UniProtKB)

Required in the nephrocyte for normal uptake of proteins and elimination of toxins, and for maintenance of endocytic trafficking structures. May function together with Cubn.

(UniProt, Q9VPN2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Amnionless for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Amnionless-RA

FBtr0078134

NM_134671

1681

505

Amnionless-RB

FBtr0330647

NM_001272884

1893

505

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Amnionless-PA

FBpp0077793

56.9

505

4.48

NP_608515

AAF51514

Amnionless-PB

FBpp0303497

56.9

505

4.48

NP_001259813

AGB92350

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

505 aa isoforms: Amnionless-PA, Amnionless-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Amnionless

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VPN2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Amnionless using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Amnionless_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

circular visceral muscle primordium

(Fisher et al., 2012)

garland cell primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

circular visceral muscle fiber

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic stage

embryonic/larval pericardial cell

(Zhang et al., 2013)

larval stage

embryonic/larval pericardial cell

(Zhang et al., 2013)

embryonic/larval garland cell

(Zhang et al., 2013)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult pericardial cell

(Hartley et al., 2016)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Amnionless protein is expressed in pericardial cells (pericardial nephrocytes).

(Hartley et al., 2016)

Marker for

pericardial cell

(Hartley et al., 2016)

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Amnionless in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Amnionless

View exonic expression by tissue for Dmel\Amnionless

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025686

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0025686

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11592-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0025686

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Amnionless

Transgenic constructs containing regulatory region of Amnionless

Deletions and Duplications ( 1 )

Disrupted in

Df(2L)Exel8001

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Amnionless^GD781

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Amnionless^GD781

viable, with Scer\GAL4^Mef2.PR

Amnionless^GD781

viable, with Scer\GAL4^tin.CΔ4

Amnionless^GD781

viable, with Scer\GAL4^Ugt36A1.PK

Amnionless^UAS.cZa

Other Phenotypes

Allele

chemical sensitive, with Scer\GAL4^Ugt36A1.PK

Amnionless^UAS.cZa

pain response defective | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Amnionless^GD781

short lived, with Scer\GAL4^Ugt36A1.PK

Amnionless^HMS02353

some die during pupal stage, with Scer\GAL4^pnr-MD237

Amnionless^GD781

visible, with Scer\GAL4^pnr-MD237

Amnionless^GD781

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^Ugt36A1.PK

Amnionless^KK103998

adult pericardial cell, with Scer\GAL4^Ugt36A1.PK

Amnionless^HMS02353

chaeta, with Scer\GAL4^pnr-MD237

Amnionless^GD781

embryonic/larval pericardial cell, with Scer\GAL4^Ugt36A1.PK

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\AMN

11 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Amn

10 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Amn

10 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\amn

7 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\amn

5 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\Y75B7AL.2

3 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905RH )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA32903

Drosophila persimilis

Dper\GL25825

Drosophila willistoni

Dwil\GK14824

Drosophila virilis

Dvir\GJ25971

Drosophila mojavensis

Dmoj\GI15029

Drosophila grimshawi

Dgri\GH10219

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503VT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010405

Glossina morsitans

Tsetse fly

Gmor\GMOY003600

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03938

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005223

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0MX7 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012714

Danaus plexippus

Monarch butterfly

Dple\DPOGS210073-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL004945

Apis florea

Little honeybee

Aflo\2561786932

Apis mellifera

Western honey bee

Amel\GB41552

Apis mellifera

Western honey bee

Amel\GB47929

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11559

Bombus terrestris

Buff-tailed bumblebee

Bter\15207140

Linepithema humile

Argentine ant

Lhum\LH20781

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0014f1

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009271

Pediculus humanus

Human body louse

Phum\PHUM422850

Cimex lectularius

Bed bug

Clec\CLEC012574

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003658

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01939

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01938

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0N60 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW007271

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81070.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g00220

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_254308

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G11HV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Amn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-AmnL

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000023032

Gallus gallus

Domestic chicken

Ggal\AMN

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Amnionless^KK103998

model of cardiomyopathy

CEA

(Hartley et al., 2016)

model of kidney disease

CEA

(Zhang et al., 2013)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AMN; amnion associated transmembrane protein

11 of 15

605799

IMERSLUND-GRASBECK SYNDROME 2; IGS2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0025686

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33199

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-0.5

Cytogenetic map

21B7-21B7

Sequence location

2L:287,252..289,144 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

21B7-21B7

Limits computationally determined from genome sequence between P{lacW}spen^k06805&P{lacW}l(2)k13604^k13604 and P{lacW}U2af38^k14504

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-0.5

(FlyBase, 2016)

2-0.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

85363

w¹¹¹⁸; PBac{PB}Amnionless^c03503

41956

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02353}attP2/TM3, Sb¹

VDRC

v104099

P{KK103998}VIE-260B

v2495

w¹¹¹⁸; P{GD781}v2495/TM3

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP03221

Other clones

DPiM_IP03221

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025686

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025686

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33199

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ivy et al., 2015)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Amnionless CG11592

(Zhang et al., 2013)

Source for database merge of

Source for merge of: BEST:CK02467 CG11592

(Bayraktaroglu, 2000.11.2)

Additional comments

Other Comments

Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

(Kopczynski et al., 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 26 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33199

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information