FlyBase Allele Report: Dmel\Amnionless[3]

General Information

Symbol

Dmel\Amnionless³

Species

D. melanogaster

Name

FlyBase ID

FBal0371574

Feature type

allele

Associated gene

Dmel\Amnionless

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

loss of function allele

(Atienza-Manuel et al., 2021)

Mutagen

CRISPR/Cas9

(Atienza-Manuel et al., 2021)

Progenitor genotype

Cytology

Description

Deletion/insertion producing a frameshift followed by a premature stop codon, resulting in expression of a truncated Amnionless protein lacking the intracellular, transmembrane and most of the extracellular domains (73 wild type amino acids, followed by 17 novel amino acids).

(Atienza-Manuel et al., 2021)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:288,700..288,701 [-]

Comment:

AG is replaced with GTCCCA, causing a frameshift at Amnionless amino acid 73 and leading to early translation termination after the addition of 17 novel amino acids.

Inserted_sequence:

GTCCCA

(Atienza-Manuel et al., 2021)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Atienza-Manuel et al., 2021)

Phenotype Manifest In

nephrocyte | larval stage

(Atienza-Manuel et al., 2021)

nephrocyte diaphragm | larval stage

(Atienza-Manuel et al., 2021)

nucleus | larval stage

(Atienza-Manuel et al., 2021)

Detailed Description

Statement

Reference

Amnionless³ homozygous larval nephrocytes exhibit a decrease in the number of slit diaphragms in the cortical region, and are not strictly confined to the entrance of labyrinthine channels but are also found at internal ectopic positions; they also show acentric nuclei.

(Atienza-Manuel et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Amnionless³ has nephrocyte diaphragm | larval stage phenotype, non-enhanceable by Cubn2^E3-1/Cubn2^E3-1

(Atienza-Manuel et al., 2021)