FlyBase Allele Report: Dmel\msi[UAS.Tag:ADARcd]

General Information

Symbol

Dmel\msi^{UAS.Tag:ADARcd}

Species

D. melanogaster

Name

FlyBase ID

FBal0373153

Feature type

allele

Associated gene

Dmel\msi

Associated Insertion(s)

Carried in Construct

P{UAS-Msi.AdarCD}

Key Links

Transgenic product class

dominant_negative_variant

(Landínez-Macías et al., 2021)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Landínez-Macías et al., 2021)

Mutagen

in vitro construct

(Landínez-Macías et al., 2021)

Progenitor genotype

Carried in construct

P{UAS-Msi.AdarCD}

Cytology

Description

UAS regulates expression of full-length msi with the catalytic domain of the RNA-editing enzyme Adar (Tag:ADARcd) fused to the C terminus. In vivo, it behaves as a dominant-negative protein.

(Landínez-Macías et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

msi

Tagged with

Tag:ADARcd

proximity labeling tool (RNA)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | cell autonomous, with Scer\GAL4^Or10a.PF

(Landínez-Macías et al., 2021)

Phenotype Manifest In

adult olfactory receptor neuron | cell autonomous, with Scer\GAL4^Or10a.PF

(Landínez-Macías et al., 2021)

antennal lobe glomerulus VA7m | cell autonomous, with Scer\GAL4^Or10a.PF

(Landínez-Macías et al., 2021)

axon | adult stage | cell autonomous, with Scer\GAL4^Or10a.PF

(Landínez-Macías et al., 2021)

Detailed Description

Statement

Reference

The expression of msi^{UAS.Tag:ADARcd} under the control Scer\GAL4^Or10a.PF results in the axons of or10a-expressing ORNs mistargeting to the VA7m glomerulus.

(Landínez-Macías et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Or10a.PF, msi^{UAS.Tag:ADARcd} has abnormal neuroanatomy | cell autonomous | adult stage phenotype, suppressible | partially by Ptp69D^UAS.Tag:MYC, Scer\GAL4^Or10a.PF

(Landínez-Macías et al., 2021)

Phenotype Manifest In