FB2026_03 , released September 17, 2026
Allele: Dmel\Dscam1TM1-2.iso-KO
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General Information
Symbol
Dmel\Dscam1TM1-2.iso-KO
Species
D. melanogaster
Name
FlyBase ID
FBal0374576
Feature type
allele
Associated gene
Dmel\Dscam1
Associated Insertion(s)
TI{TI}Dscam1TM1-2.iso-KO
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Liu et al., 2020)
gene targeting by homologous recombination
(Liu et al., 2020)
Progenitor genotype
Associated Insertion(s)
TI{TI}Dscam1TM1-2.iso-KO
Cytology
Description

An 'isoTarget knockout' (iso-KO) cassette has been inserted into the endogenous Dscam1 locus, in the juxtamembrane domain in exon 17.1. Exons 17.1 and 17.2 of Dscam1 are mutually exclusive, encoding two different transmembrane and juxtamembrane domains (TM1 and TM2); exon 17.1 encodes the TM1 alternative form. The iso-KO cassette consists of 1. an RSRT stop cassette containing multiple termination codons to ensure disruption of translation and 2. a Tag:HA epitope. In the absence of RSR recombinase, this was expected to result in loss of function of only TM1-containing isoforms of Dscam1, however expression of TM2-containing isoforms is also affected in this allele.

(Liu et al., 2020)
Allele components
Component
Use(s)
Also carries
RSRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dscam1TM1-2.iso-KO
      Dscam[TM1/2]iso-KO
      (Liu et al., 2020)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)