FlyBase Allele Report: Hsap\APOL1[G0.UAS.Tag:HA]

General Information

Symbol

Hsap\APOL1^{G0.UAS.Tag:HA}

Species

H. sapiens

Name

FlyBase ID

FBal0374918

Feature type

allele

Associated gene

Hsap\APOL1

Associated Insertion(s)

Carried in Construct

M{UAS-hAPOL1.G0.HA}

Key Links

Transgenic product class

cDNA

(Gerstner et al., 2022)

wild_type

(Gerstner et al., 2022)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gerstner et al., 2022)

wild_type

(Gerstner et al., 2022)

Mutagen

in vitro construct

(Gerstner et al., 2022)

Progenitor genotype

Carried in construct

M{UAS-hAPOL1.G0.HA}

Cytology

Description

UAS regulatory sequences drive expression of a full-length, wild type (G0) Hsap\APOL1 cDNA (GenBank ID BC112943.1). The coding sequence is tagged with Tag:HA.

(Gerstner et al., 2022)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\APOL1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | third instar larval stage, with Scer\GAL4^ptc.PU

(Gerstner et al., 2022)

lethal, with Scer\GAL4^pros.PU

(Gerstner et al., 2022)

lethal, with Scer\GAL4^Ugt36A1.PU

(Gerstner et al., 2022)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc.PU

(Gerstner et al., 2022)

Phenotype Manifest In

embryonic/larval garland cell | third instar larval stage, with Scer\GAL4^Hand.PU

(Gerstner et al., 2022)

endoplasmic reticulum | third instar larval stage, with Scer\GAL4^Hand.PU

(Gerstner et al., 2022)

wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

(Gerstner et al., 2022)

Detailed Description

Statement

Reference

The expression of Hsap\APOL1^{G0.UAS.Tag:HA} under the control of Scer\GAL4^Hand.PU induces ER swelling in third instar larval garland cell nephrocytes.

The expression of Hsap\APOL1^{G0.UAS.Tag:HA} under the control of Scer\GAL4^ptc.PU induces cell death in the third instar larval wing disc.

(Gerstner et al., 2022)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\APOL1^{G0.UAS.Tag:HA}, Scer\GAL4^ptc.PU has increased cell death | third instar larval stage phenotype, suppressible by Xbp1^HMS03015, Scer\GAL4^ptc.PU

(Gerstner et al., 2022)

Hsap\APOL1^{G0.UAS.Tag:HA}, Scer\GAL4^ptc.PU has lethal - all die before end of pupal stage phenotype, suppressible by Xbp1^HMS03015, Scer\GAL4^ptc.PU

(Gerstner et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\APOL1^{G0.UAS.Tag:HA}, Scer\GAL4^ptc.PU has wing disc | third instar larval stage phenotype, suppressible by Xbp1^HMS03015, Scer\GAL4^ptc.PU

(Gerstner et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Hsap\APOL1^{G0.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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