A TI{CRIMIC.TG4.2} DNA cassette has been inserted into Dic3, in a coding intron, and is predicted to gene trap a subset of the annotated transcripts of the gene (the inserted sequence is in the correct phase to trap the phase 2 Dic3-RA transcript, but is wrong for trapping the phase 1 Dic3-RB transcript). The TI{CRIMIC.TG4.2} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: GATGAAGACAAACGAACAGATGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.2} cassette is predicted to be accompanied by a deletion of 2bp of genomic sequence flanking the insertion site.