A TI{KozakGAL4} DNA cassette has been inserted into Oli, replacing the coding sequence (coordinates of deleted sequence are 2L:17589554..17590984 , release 6 genome). This results in a simultaneous knock-out of Oli plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Oli (predicted to gene trap all annotated transcripts of the gene). The insertion is also within lncRNA:CR44410 and is not predicted to gene trap this gene. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ACAAGTTCGCCGGTGTACGAAGG and ATTTAACTGCTGCTAGTCGGTGG. The PCR check gave the expected-sized band for the right flank and shorter than expected band for the left flank. The right insertion site was verified by sequencing.