FlyBase Allele Report: Dmel\Rap1[Q63E.UASp.GFP]

General Information

Symbol

Dmel\Rap1^{Q63E.UASp.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0375864

Feature type

allele

Associated gene

Dmel\Rap1

Associated Insertion(s)

Carried in Construct

P{UASp-GFP.Rap1.Q63E}

Also Known As

Rap1-CA

Key Links

Transgenic product class

missense_variant

(Bonello et al., 2018, Ellis et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Bonello et al., 2018, Ellis et al., 2013)

Mutagen

in vitro construct

(Bonello et al., 2018, Ellis et al., 2013)

Progenitor genotype

Carried in construct

P{UASp-GFP.Rap1.Q63E}

Cytology

Description

UASp regulatory sequences drive expression of Rap1 that has been mutated so that it is constitutively GTP-bound and thus active. The protein is tagged at the N-terminal end with GFP.

(Bonello et al., 2018)

UASp regulatory sequences drive expression of a constitutively active form of Rap1 containing a Q63E amino acid replacement.

(Ellis et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rap1

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | dorsal closure stage, with Scer\GAL4^c381

(Ellis et al., 2013)

Detailed Description

Statement

Reference

Expression of R^{Q63E.Scer\UAS.P\T} in the amnioserosa under the control of Scer\GAL4^c381 results in dorsal closure defects. More than 60% of embryos have open dorsal holes at the end of stage 15 with about 20% of embryos failing to complete dorsal closure altogether. Embryos exhibit greater integrin recruitment to the lateral membrane of amnioserosa (AS) cells.

(Ellis et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rap1^{Q63E.UASp.GFP}, Scer\GAL4^lz-gal4 is a suppressor of visible | adult stage phenotype of Scer\GAL4^lz-gal4, emc^KK108316

(Reddy Onteddu et al., 2024)

Rap1^{Q63E.UASp.GFP}, Scer\GAL4^lz-gal4 is a suppressor of visible | adult stage phenotype of Scer\GAL4^lz-gal4, da^{da-da.cWa.UAS}