FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\Lkb1KI.T353A
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General Information
Symbol
Dmel\Lkb1KI.T353A
Species
D. melanogaster
Name
FlyBase ID
FBal0389323
Feature type
allele
Associated gene
Dmel\Lkb1
Associated Insertion(s)
TI{TI}Lkb1KI.T353A
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Borkowsky et al., 2023)
gene targeting by homologous recombination
(Borkowsky et al., 2023)
Progenitor genotype
Associated Insertion(s)
TI{TI}Lkb1KI.T353A
Cytology
Description

A T353A amino acid replacement (non-phosphorylatable mutation) has been introduced into the Lkb1 coding sequence. A loxP cassette containing the Disc\RFPDsRed(T1).3xP3.cHa marker has been inserted during the mutagenesis event.

(Borkowsky et al., 2023)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:13,678,166..13,678,166 [+]
Nucleotide change:

A13678166G

Amino acid change:

T353A | Lkb1-PA; T353A | Lkb1-PB; T353A | Lkb1-PC; T353A | Lkb1-PD; T353A | Lkb1-PE; T353A | Lkb1-PF; T353A | Lkb1-PH; T353A | Lkb1-PI; T353A | Lkb1-PJ

Reported amino acid change:

T353A

Comment:

A T353A amino acid replacement has been introduced into the Lkb1 coding sequence. A loxP cassette containing the Disc\RFPDsRed(T1).3xP3.cHa marker has been inserted during the mutagenesis event. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Borkowsky et al., 2023)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Lkb1KI.T353A
      Name Synonyms
      Secondary FlyBase IDs
        References (1)