FlyBase Allele Report: Dmel\Shmt[m3-5]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Shmt^m3-5

General Information

Symbol

Dmel\Shmt^m3-5

Species

D. melanogaster

Name

FlyBase ID

FBal0391868

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

Allele class

loss of function allele

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

loss of function allele

(Silva et al., 2023)

Mutagen

(Silva et al., 2023)

Progenitor genotype

Cytology

Description

4bp deletion in exon 3 that is predicted to result in a frameshift from amino acid 120 that introduces a premature stop codon at amino acid 132, affecting all Shmt isoforms.

(Silva et al., 2023)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

X:5,914,237..5,914,240 [-]

Comment:

A 4bp deletion in Shmt leading to a frameshift after amino acid 120 of Shmt-RB and Shmt-RB, and leading to early translation termination after the addition of 12 novel amino acids.

(Silva et al., 2023)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | larval stage

(Silva et al., 2023)

decreased size | larval stage | somatic clone

(Silva et al., 2023)

increased cell death | larval stage

(Silva et al., 2023)

increased cell size | larval stage

(Silva et al., 2023)

lethal - all die before end of P-stage | male

(Silva et al., 2023)

some die during P-stage | male

(Silva et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Shmt^m3-5 has decreased size | larval stage | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA

(Silva et al., 2023)

Shmt^m3-5 has decreased size | larval stage | somatic clone phenotype, non-enhanceable by BacA\p35^UAS.cUa/Scer\GAL4^Tub.PU

(Silva et al., 2023)

NOT suppressed by

Statement

Reference

Shmt^m3-5 has decreased size | somatic clone | larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA

(Silva et al., 2023)

Shmt^m3-5 has decreased size | somatic clone | larval stage phenotype, non-suppressible by BacA\p35^UAS.cUa/Scer\GAL4^Tub.PU

(Silva et al., 2023)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Shmt^m3-5 has optic lobe neuroepithelial cell | larval stage | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA

(Silva et al., 2023)

Shmt^m3-5 has optic lobe neuroepithelial cell | larval stage | somatic clone phenotype, non-enhanceable by BacA\p35^UAS.cUa/Scer\GAL4^Tub.PU

(Silva et al., 2023)

NOT suppressed by

Statement

Reference

Shmt^m3-5 has optic lobe neuroepithelial cell | somatic clone | larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA

(Silva et al., 2023)

Shmt^m3-5 has optic lobe neuroepithelial cell | somatic clone | larval stage phenotype, non-suppressible by BacA\p35^UAS.cUa/Scer\GAL4^Tub.PU

(Silva et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Shmt^m3-5 is rescued by Shmt^+t4.9

(Silva et al., 2023)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Shmt^m3-5

shmt^m3-5

(Silva et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections