FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\CG13321G4-N-int
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General Information
Symbol
Dmel\CG13321G4-N-int
Species
D. melanogaster
Name
FlyBase ID
FBal0392638
Feature type
allele
Associated gene
Dmel\CG13321
Associated Insertion(s)
TI{2A-GAL4(1-20)::N-int}CG13321G4-N-int
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Ewen-Campen et al., 2023)
gene targeting by homologous recombination
(Ewen-Campen et al., 2023)
Progenitor genotype
Associated Insertion(s)
TI{2A-GAL4(1-20)::N-int}CG13321G4-N-int
Cytology
Description

A TI{2A-GAL4(1-20)::N-int} cassette has been inserted in-frame into the endogenous CG13321 locus. The presence of the 2A peptide results in the GAL4(1-20)::N-int hemidriver being expressed as a separate protein under the control of endogenous CG13321 regulatory sequences. The TI{2A-GAL4(1-20)::N-int} cassette was inserted via the CRISPR/Cas-9 drop-in technique. The sgRNA sequence used to target the gene was: CCAGCTAGGATGGCTCCTGG.

(Ewen-Campen et al., 2023)
Allele components
Component
Use(s)
Inserted element
TI{2A-GAL4(1-20)::N-int}
Encoded product / tool
GAL4(1-20)::N-int
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CG13321G4-N-int
      Name Synonyms
      Secondary FlyBase IDs
        References (1)