A Tag:HA peptide tag flanked by flexible linkers and a cassette encoding a visible marker flanked by piggyBac inverted terminal repeat sequences are inserted immediately after the sole fid translational start site. The visible marker interfering cassette (IC) disrupts the open reading frame to generate a null allele due to stop codons in the inverted repeat sequences.
fidHA+IC/fidKO is rescued by fidWT.UAS/Scer\GAL4how-24B
fidHA+IC/fidKO is partially rescued by Scer\GAL4how-24B/fidMTD.UAS
fidHA+IC/fidKO is not rescued by fidWT.UAS/Scer\GAL4elav-C155