UASp regulates expression of wild type Acly cDNA (FBpp0289823) tagged with 3xTag:HA before the stop codon. The sequence targeted by P{TRiP.HMC06049} is mutated to make the UAS line resistant to the shRNA (CACGACCTATGTA- GACCTGTA > TACAACGTACGTGGATTTATA, 8 mutations introduced without affecting the Acly protein sequence).