UASp regulates expression of Acly cDNA (FBpp0289823) containing a D1038>A (CoA-binding-dead) variant, and tagged with 3xTag:HA before the stop codon. The sequence targeted by P{TRiP.HMC06049} is mutated to make the UAS line resistant to the shRNA (CACGACCTATGTA- GACCTGTA > TACAACGTACGTGGATTTATA, 8 mutations introduced without affecting the Acly protein sequence).
AclyD1038A.UASp.Tag:HA/Scer\GAL4bam.PU fails to rescue AclyHMC06049