dsRed has been inserted in the intronic region before the preserved splice site of exon 7A. After the preserved splice site, the next 3 amino acids were also preserved before introducing an early stop codon flanked by restriction enzyme sites.

GATCTTTACTAGTGCTCTTCTATAACTTCGTATAGCATACATTATACGAAGTTATACCGGTTAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCTAGAGTCGCGGCCCCTACAGGAACAGGTGGTGGCGGCCCTCGGCGCGCTCGTACTGCTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAGCTTGGAGTCCACGTAGTAGTAGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGATGGACTTGAACTCCACCAGGTAGTGGCCGCCGTCCTTCAGCTTCAGGGCCTTGTGGATCTCGCCCTTCAGCACGCCGTCGCGGGGGTACAGGCGCTCGGTGGAcGCCTCCCAGCCCATAGTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTCACGCCGATGAACTTCACCTTGTAGATGAAGGAGCCGTCCTGGAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGTCCTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCTTGTAGTCGGGGATGTCGGCGGGGTGCTTCACGTACACCTTGGAGCCGTACTGGAACTGGGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACGTCCTCGGAGGAGGCCATGGTGGCGACCGGCTTCGAGCCGATTGTTTAGCTTGTTCAGCTGCGCTTGTTTATTTGCTTAGCTTTCGCTTAGCGACGTGTTCACTTTGCTTGTTTGAATTGAATTGTCGCTCCGTAGACGAAGCGCCTCTATTTATACTCCGGCGGTCGAGGGTTCGAAATCGATAAGCTTGGATCCTAATTGAATTAGCTCTAATTGAATTAGTCTCTAATTGAATTAGATCCCGTACGATAACTTCGTATAGCATACATTATACGAAGTTATCTACGCCCCCAACTGAGAGAACTCAAAGGTTACCCCAGTTGGGGCACTACGATCGCAGGTGTGCATATGTCCGCGGTTTCTTTTACAGAACTGAAAAATCATATGTAACTAACTAACCTAAGG