An early stop codon is inserted at the beginning of cpx exon 7B.
dsRed has been inserted in the intronic region before the preserved splice site of exon 7B. After the preserved splice site, the next 3 amino acids were also preserved before introducing an early stop codon flanked by restriction enzyme sites.
GATCTTTACTAGTGCTCTTCTATAACTTCGTATAGCATACATTATACGAAGTTATACCGGTTAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCTAGAGTCGCGGCCCCTACAGGAACAGGTGGTGGCGGCCCTCGGCGCGCTCGTACTGCTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATGTCCAGCTTGGAGTCCACGTAGTAGTAGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGATGGACTTGAACTCCACCAGGTAGTGGCCGCCGTCCTTCAGCTTCAGGGCCTTGTGGATCTCGCCCTTCAGCACGCCGTCGCGGGGGTACAGGCGCTCGGTGGAcGCCTCCCAGCCCATAGTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTCACGCCGATGAACTTCACCTTGTAGATGAAGGAGCCGTCCTGGAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGTCCTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCTTGTAGTCGGGGATGTCGGCGGGGTGCTTCACGTACACCTTGGAGCCGTACTGGAACTGGGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACGTCCTCGGAGGAGGCCATGGTGGCGACCGGCTTCGAGCCGATTGTTTAGCTTGTTCAGCTGCGCTTGTTTATTTGCTTAGCTTTCGCTTAGCGACGTGTTCACTTTGCTTGTTTGAATTGAATTGTCGCTCCGTAGACGAAGCGCCTCTATTTATACTCCGGCGGTCGAGGGTTCGAAATCGATAAGCTTGGATCCTAATTGAATTAGCTCTAATTGAATTAGTCTCTAATTGAATTAGATCCCGTACGATAACTTCGTATAGCATACATTATACGAAGTTATCTACGCCCCCAACTGAGAGAACTCAAAGGTTACCCCAGTTGGGGCACTACGATCGCAGGTGTGCATATGTCCGCGGTTGTATTTTCAGCAAAGTTGAAACATATGTAACTAACTAACCTAAGGTTGAAA
