FB2026_02 , released June 18, 2026
Allele: Dmel\TetCD.EGFP
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General Information
Symbol
Dmel\TetCD.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0398879
Feature type
allele
Associated gene
Dmel\Tet
Associated Insertion(s)
TI{TI}TetCD.EGFP
Carried in Construct
Also Known As
tetCD-GFP
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Boulet et al., 2023)
gene targeting by homologous recombination
(Boulet et al., 2023)
Progenitor genotype
Associated Insertion(s)
TI{TI}TetCD.EGFP
Cytology
Description

Two mutations have been introduced into the endogenous Tet locus (amino acid replacements H1947Y and D1949A), resulting in a catalytically dead protein. In addition, an EGFP tag has been inserted at the C-terminal end of the open reading frame.

(Boulet et al., 2023)
Allele components
Component
Use(s)
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:2,791,624..2,791,624 [-]
Nucleotide change:

A2791624C

Amino acid change:

D1888A | Tet-PA; D1943A | Tet-PB; D1084A | Tet-PC; D1084A | Tet-PD; D1894A | Tet-PE; D1949A | Tet-PF

Reported amino acid change:

D1949A

Comment:

Two mutations have been introduced into the endogenous Tet locus (amino acid replacements H1947Y and D1949A), resulting in a catalytically dead protein.

(Boulet et al., 2023)
point_mutation
3L:2,791,631..2,791,631 [-]
Nucleotide change:

C2791631T

Amino acid change:

H1886Y | Tet-PA; H1941Y | Tet-PB; H1082Y | Tet-PC; H1082Y | Tet-PD; H1892Y | Tet-PE; H1947Y | Tet-PF

Reported amino acid change:

H1947Y

Comment:

Two mutations have been introduced into the endogenous Tet locus (amino acid replacements H1947Y and D1949A), resulting in a catalytically dead protein.

(Boulet et al., 2023)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TetCD.EGFP
      TetCD
      (Gerdy et al., 2025)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)