FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ncra\QFCG9458-2A-QF2
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General Information
Symbol
Ncra\QFCG9458-2A-QF2
Species
N. crassa
Name
FlyBase ID
FBal0401837
Feature type
allele
Associated gene
Ncra\QF
Associated Insertion(s)
TI{2A-QF2}CG94582A-QF2
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Zirin et al., 2024)
gene targeting by homologous recombination
(Zirin et al., 2024)
Progenitor genotype
Associated Insertion(s)
TI{2A-QF2}CG94582A-QF2
Cytology
Description

A sequence cassette encoding T2A-QF2 (with an Hsp70 terminator sequence) followed by a loxP cassette containing a Disc\RFPDsRed.3xP3.cUa marker has been inserted into the endogenous CG9458 locus. The cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TTTCGATTCCGCATGATCTT.

(Zirin et al., 2024)
Allele components
Component
Use(s)
Inserted element
TI{2A-QF2}
Encoded product / tool
QF2
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ncra\QFCG9458-2A-QF2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)