The loxP cassette in TI{2A-QF2}phtm2A-QF2 has been excised, removing the Disc\RFPDsRed.3xP3.cUa marker. This leaves sequence encoding T2A-QF2 (with an Hsp70 terminator sequence) followed by a single loxP site, inserted into the endogenous phtm locus.