A TI{KozakGAL4} DNA cassette has been inserted into Nfs1, replacing the coding sequence (coordinates of deleted sequence are 2L:11999705..12001194 , release 6 genome). This results in a simultaneous knock-out of Nfs1 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Nfs1 (predicted to gene trap all annotated transcripts of the gene). The 3' end of the deletion associated with the insertion is 40 bp downstream of the 3' end of the Hacd1 gene and may affect the proper 3' end formation of its transcripts. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: TGATGGAATCCTAGAAATTCTGG and AAAGTGTGGTGTAACAGTAACGG.