A sequence cassette encoding 1. four copies of sequence encoding the mNG2(11) and Tag:OLLAS tags, 2. a 2A linker and 3. the QF2 driver has been inserted into the endogenous Eato locus. This results in Eato being tagged with mNG2(11) and Tag:OLLAS, and the presence of the 2A sequence allows separate translation of the QF2 driver under the control of Eato regulatory sequences.