A TI{KozakGAL4} DNA cassette has been inserted into CAH3, replacing the coding sequence (coordinates of deleted sequence are X:8686721..8688192 , release 6 genome). This results in a simultaneous knock-out of CAH3 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of CAH3 (predicted to gene trap a subset of the annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: AAGCGGGCTTAACCAATCCGTGG and AGAATTGTATCCAAGTCATATGG.