FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Ncra\QFQF2.Ubi.DQVD
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General Information
Symbol
Ncra\QFQF2.Ubi.DQVD
Species
N. crassa
Name
FlyBase ID
FBal0409458
Feature type
allele
Associated gene
Ncra\QF
Associated Insertion(s)
Carried in Construct
P{Ubi(-FRT)mCD8::DQVD::QF2}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Muramoto et al., 2025)
Progenitor genotype
Carried in construct
P{Ubi(-FRT)mCD8::DQVD::QF2}
Cytology
Description

The FRT stop cassette has been excised, allowing the ubiquitin promoter to drive expression of a fusion protein composed of a transmembrane domain (Tag:M(mCd8a)), a DQVD caspase cleavage sequence, and QF2. This protein is designed to act as a caspase-inducible form of QF2. In the absence of caspase, the transmembrane domain tethers QF2 to the plasma membrane, rendering it inactive. In the presence of caspase, the fusion protein is cleaved at the DQVD site, releasing the QF2 coding region so that in can enter the nucleus and drive transcription.

(Muramoto et al., 2025)
Allele components
Component
Use(s)
Encoded product / tool
QF2
Tagged with
Tag:M(mCd8a)
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ncra\QFQF2.Ubi.DQVD
      Name Synonyms
      Secondary FlyBase IDs
        References (1)