FB2026_03 , released September 17, 2026
Recombinant Construct: P{Ubi(-FRT)mCD8::DQVD::QF2}
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General Information
Symbol
P{Ubi(-FRT)mCD8::DQVD::QF2}
FlyBase ID
FBtp0173775
Feature type
transgenic_transposable_element
Component Allele(s)
Ncra\QFQF2.Ubi.DQVD
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ncra\QFQF2.Ubi.DQVD
Transgenic product class
Component
Use(s)
Encoded product / tool
QF2
Tagged with
Tag:M(mCd8a)
Also carries
FRT
Description

The FRT stop cassette has been excised, allowing the ubiquitin promoter to drive expression of a fusion protein composed of a transmembrane domain (Tag:M(mCd8a)), a DQVD caspase cleavage sequence, and QF2. This protein is designed to act as a caspase-inducible form of QF2. In the absence of caspase, the transmembrane domain tethers QF2 to the plasma membrane, rendering it inactive. In the presence of caspase, the fusion protein is cleaved at the DQVD site, releasing the QF2 coding region so that in can enter the nucleus and drive transcription.

(Muramoto et al., 2025)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{Ubi(-FRT)mCD8::DQVD::QF2}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)