FlyBase Allele Report: Dmel\Ras85D[V12.ΔCAAX.sev.Tag:Myr(Src64B)]

General Information

Symbol

Dmel\Ras85D^{V12.ΔCAAX.sev.Tag:Myr(Src64B)}

Species

D. melanogaster

Name

FlyBase ID

FBal0410721

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{Ras^Myr}

P{sev-Src90-Ras1^V12.ΔCAAX}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Therrien et al., 1995)

Progenitor genotype

Carried in construct

P{Ras^Myr}

P{sev-Src90-Ras1^V12.ΔCAAX}

Cytology

Description

sev enhancer and promoter sequences drive expression of Ras85D carrying two mutations: a G12V amino acid replacement and deletion of the C-terminal CAAX box. The coding sequence is tagged at the N-terminal end with Tag:Myr(Src64B) (which contains a myristoylation signal).

(Therrien et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

sev

Encoded product / tool

Ras85D

Tagged with

Tag:Myr(Src64B)

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye

(Karim et al., 1996, Therrien et al., 1995)

photoreceptor cell R7

(Kauffmann et al., 1995, Therrien et al., 1995)

Detailed Description

Statement

Reference

Rough eye phenotype similar to Ras85D^V12.sev.

(Karim et al., 1996)

Severe roughening of the eye external surface, section through an eye reveals multi R7 cells per ommatidium. Injection of a Ras CAAX peptidomimetic, FTI-254, does not rescue the mutant eye phenotype but slightly reduces the average number of R7 cells per ommatidium.

(Kauffmann et al., 1995)

Same phenoytpe as Ras85D^V12.sev : extra R7 cells and rough eyes.

(Therrien et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^{V12.ΔCAAX.sev.Tag:Myr(Src64B)} has phenotype, enhanceable by aop^E-867

(Karim et al., 1996)

Ras85D^{V12.ΔCAAX.sev.Tag:Myr(Src64B)} has phenotype, enhanceable by aop^yan-XE18

(Karim et al., 1996)

Suppressed by

Statement

Reference

Ras85D^{V12.ΔCAAX.sev.Tag:Myr(Src64B)} has eye phenotype, suppressible by ksr^S-638

(Therrien et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Phenotype is suppressed by ksr mutants but not by βggt-I mutants.

(Therrien et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras85D::Src64B^Myr.sev

Ras85D::Src64B^{V12.ΔCAAX.sev}

Ras85D^{V12.ΔCAAX.sev.Tag:Myr(Src64B)}

Ras^Myr

(Kauffmann et al., 1995)

Name Synonyms

Secondary FlyBase IDs

FBal0048922
FBal0048921

References (4)

Report Sections

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